GENETIC ENGINEERING AND BIOTECHNOLOGY

Unit I

GENETIC ENGINEERING

Restriction enzymes: discovery

Restriction enzymes:nomenclature

Restriction enzymes:types

Restriction enzymes: uses

Linking of DNA- ligases

Linking of DNA- linkers

Linking of DNA-adaptors

Linking of DNA-Homopolymer tails

Unit II

Gene transfer techniques

Calcium phosphate coprecipitation

transduction

protoplast fusion

electroporation

Microinjection

lipofection

Selection and Screening

Insertional inactivation Immunological screening

Insertional inactivation Immunological screening

DNA Hybridization

Northern, Southern, Western Blotting and PCR

Northern, Southern, Western Blotting and PCR

Unit III

Biotechnology: Definition

Scope

Biotechnology as an interdisciplinary pursuit

Plant Biotechnology

Callus Culture

micropropagation

protoplast culture

Cloning of disease resistant plants

cloning of Bacillus thuringiensis

crystal protein gene in plants

crystal protein gene in plants

Nif gene cloning

Application of plant tissue culture

Microbial Biotechnology

SCP and its applications

Unit IV

ANIMAL BIOTECHNOLOGY

Animal cell culture

culture media

primary and continuous culture

cell lines and its applications

lymphocyte culture

Stem cells and its applications

Unit V

Fish Biotechnology

ploidy induction

Transgenic fish

IVF

embryo transfer

Transgenic live stock production and  application

Transgenic live stock production and  application

Knockout mice

Knockout mice

Rules in Biotechnology

Patent (IPR)